Hannah Geiser, Goshen College

Class Year:

2012

Mentor:

Alyson Santoro, Ph.D.

Location:

Project Title:

Presence of Ammonia-Oxidizing Archaea and Bacteria along Physiochemical Gradients in Coastal Beaches and Estuaries

Abstract:

Coastal intertidal estuaries in California and Maryland were analyzed for the presence of ammonia-oxidizing archaea and bacteria and correlation with dissolved inorganic nitrogen removal. DNA was extracted from sediment samples and real-time polymerase chain reaction was used to quantify the presence of the amoA gene subunit, a portion of the gene that encodes for the conversion of ammonia to hydroxylamine. Ammonia-oxidizing archaea and bacteria were found with relatively more archaea in the coastal sediments. The abundance of ammonia-oxidizing bacteria appeared to correlate with dissolved oxygen. Removal of dissolved inorganic nitrogen did not appear to be limited by the abundance of microbes found in the sediment.

Presentations

Geiser, H.*, N. de Sieyes, A. Boehm, and A. Santoro. 2013. Distribution of ammonia-oxidizing archaea and bacteria along physiochemical gradients at subterranean estuaries in California and Maryland. ASLO Aquatic Sciences Meeting, New Orleans, Louisiana.

The REU students are indicated with an asterisk (*).

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