Is Hematodinium spp. the Culprit?
Lesson Plan Standards:MD State: 1.1.1, 1.2.3, 1.2.4, 1.2.5, 1.2.6, 1.2.7, 1.3.2, 1.3.3, 1.3.4, 1.4.2, 1.4.3, 1.4.6, 1.4.9, 1.5.1, 1.5.2, 1.5.5, 1.5.9, 1.7.6, 2.1.1, 3.3.3, 3.4.2, 3.5.2, 3.5.3, 3.6.1, 6.2.3, 6.4.3, 6.4.4, 6.4.5
Lesson Plan:The Case of the Disappearing Blue Crab
Students will be provided with mock crab hemolymph samples and will use gel electrophoresis to determine if Hematodinium DNA is present. Prior to this lesson, students should have an understanding of the structure of DNA and biotechnology techniques, such as gel electrophoresis, that are used to "fingerprint" an organism.
Students will be able to use gel electrophoresis in order to determine if the Blue crab has been infected by Hematodinium.
- Case of the Crown Jewels Towson University Center for STEM Excellence (http://www.towson.edu/cse/ ) loaner kit. This kit provides the teacher with the gel electrophoresis equipment, pipettes and chemicals (including mock DNA samples). The kit can be requested at a time for a 10 day period. Teachers must have training before loaning materials from the lab. The teacher must change of the labels on the provided DNA samples to read: Positive control for Hematodinium, Negative control, Crab hemolymph 1, crab hemolymph 2, etc.
- Student Lab worksheet
- Digital camera. (optional)
- Printer (optional)
- Students will complete the lab "Is Hematodinium the Culprit" by following a series of step-by-step directions for loading and running the gel.
- Students will photograph their gel to later be printed and analyzed (optional) or sketch their results with paper and pencil.
MdBio Case of the Broken Beaker,