Science Serving Maryland's Coasts

Research Publications: UM-SG-RS-2006-18


Complete cellulase system in the marine bacterium Saccharophagus degradans strain 2-40(T).




Taylor, LE; Henrissat, B; Coutinho, PM; Ekborg, NA; Hutcheson, SW; Weiner, RA


Journal of Bacteriology 188(11):3849-3861




Saccharophagus degradans strain 2-40 is a representative of an emerging group of marine complex pollysaccharide (CP)-degrading bacteria. It is unique in its metabolic versatility, being able to degrade at least 10 distinct CPs from diverse algal, plant and invertebrate sources. The S. degradans genome has been sequenced to completion, and more than 180 open reading frames have been identified that encode carbohydrases. Over half of these are likely to act on plant cell wall polymers. In fact, there appears to be a full array of enzymes that degrade and metabolize plant cell walls. Genomic and proteomic analyses reveal 13 cellulose depolymerases complemented by seven accessory enzymes, including two cellodextrinases, three cellobiases, a cellodextrin phosphorylase, and a cellobiose phosphorylase. Most of these enzymes exhibit modular architecture, and some contain novel combinations of catalytic and/or substrate binding modules. This is exemplified by endoglucanase Ce15A, which has three internal family 6 carbohydrate binding modules (CBM6) and two catalytic modules from family five of glycosyl hydrolases (GH5) and by Ce16A, a nonreducing-end cellobiobydrolase from family GH6 with tandem CBM2s. This is the first report of a complete and functional cellulase system in a marine bacterium with a sequenced genome.

Related Research Project(s) Funded by Maryland Sea Grant: 

Maryland Sea Grant Topic(s): 

'Related Research Project(s)' link to details about research projects funded by Maryland Sea Grant that led to this publication. These details may include other impacts and accomplishments resulting from the research.

'Maryland Sea Grant Topic(s)' links to related pages on the Maryland Sea Grant website.