R/BT-10

Chitinosomes in Microbulbifer degradans: A New Paradigm for the Degradation of Complex Polysaccharides of Marine Origin

Principal Investigator:

Ronald Weiner

Start/End Year:

2002 - 2005

Institution:

University of Maryland, College Park

Co-Principal Investigator:

Steve Hutcheson, University of Maryland, College Park

Description:

1. Characterization of 2-40 chitinosomes, including protein complement, genetic regulatory sequences, protein interaction domains and operon organization. 2. Elucidation of the organization and environmental applications of chitinosomes and chitinase-containing extracellular structures (ES).

Related Publications:

Weiner, RM; Taylor, LE; Henrissat, B; Hauser, L; Land, M; Coutinho, PM; Rancurel, C; Saunders, EH; Longmire, AG; Zhang, HT; Bayer, EA; Gilbert, HJ; Larimer, F; Zhulin, IB; Ekborg, NA; Lamed, R; Richardson, PM; Borovok, I; Hutcheson, S. 2008. Complete genome sequence of the complex carbohydrate-degrading marine bacterium, Saccharophagus degradans strain 2-40(T). PLOS Genetics4(5):1 -13. doi:10.1371/journal.pgen.1000087. UM-SG-RS-2008-08.

Badger, JH; Hoover, TR; Brun, YV; Weiner, RM; Laub, MT; Alexandre, G; Mrazek, J; Ren, QH; Paulsen, IT; Nelson, KE; Khouri, HM; Radune, D; Sosa, J; Dodson, RJ; Sullivan, SA; Rosovitz, MJ; Madupu, R; Brinkac, LM; Durkin, AS; Daugherty, SC; Kothari, SP; Giglio, MG; Zhou, LW; Haft, DH; Selengut, JD; Davidsen, TM; Yang, Q; Zafar, N; Ward, NL. 2006. Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus. Journal of Bacteriology188(19):6841 -6850. doi:10.1128/JB.00111-06. UM-SG-RS-2006-24.

Ekborg, NA; Taylor, LE; Longmire, AG; Henrissat, B; Weiner, RM; Hutcheson, SW. 2006. Genomic and proteomic analyses of the agarolytic system expressed by Saccharophagus degradans 2-40. Applied and Environmental Microbiology72(5):3396 -3405. doi:10.1128/AEM.72.5.3396-3405.2006. UM-SG-RS-2006-15.

Taylor, LE; Henrissat, B; Coutinho, PM; Ekborg, NA; Hutcheson, SW; Weiner, RA. 2006. Complete cellulase system in the marine bacterium Saccharophagus degradans strain 2-40(T). Journal of Bacteriology188(11):3849 -3861. doi:10.1128/JB.01348-05. UM-SG-RS-2006-18.

Ekborg, NA; Gonzalez, JM; Howard, MB; Taylor, LE; Hutcheson, SW; Weiner, RM. 2005. Saccharophagus degradans gen. nov., sp nov., a versatile marine degrader of complex polysaccharides. International Journal of Systematic and Evolutionary Microbiology55:1545 -1549. doi:10.1099/ijs.0.63627-0. UM-SG-RS-2005-07.

Howard, MB; Ekborg, NA; Taylor, LE; Hutcheson, SW; Weiner, RM. 2004. Identification and analysis of polyserine linker domains in prokaryotic proteins with emphasis on the marine bacterium Microbulbifer degradans. Protein Science13(5):1422 -1425. doi:10.1110/ps.03511604. UM-SG-RS-2004-02.

Howard, MB; Ekborg, NA; Taylor, LE; Weiner, RM; Hutcheson, SW. 2004. Chitinase B of "Microbulbifer degradans" 2-40 contains two catalytic domains with different chitinolytic activities. Journal of Bacteriology186(5):1297 -1303. doi:10.1128/JB.186.5.1297-1303.2004. UM-SG-RS-2004-03.

Howard, MB; Ekborg, NA; Taylor, LE; Weiner, RM; Hutcheson, SW. 2003. Genomic analysis and initial characterization of the chitinolytic system of Microbulbifer degradans strain 2-40. Journal of Bacteriology185(11):3352 -3360. doi:10.1128/JB.185.11.3352-3360.2003. UM-SG-RS-2003-09.

Howard, MB; Ekborg, NA; Weiner, RM; Hutcheson, SW. 2003. Detection and characterization of chitinases and other chitin-modifying enzymes. Journal of Industrial Microbiology & Biotechnology30(11):627 -635. doi:10.1007/s10295-003-0096-3. UM-SG-RS-2003-08.

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