Behind the Scenes: Getting the shot takes teamwork

Making the invisible visible is an important, yet challenging, part of sharing science. It’s one our communications team recently took on with Assistant Director for Education J. Adam Frederick to determine the best method for capturing images of microplastics in a laboratory setting at the Institute of Marine and Environmental Technology (IMET) in Baltimore, Maryland.

Frederick is working with educators and researchers to examine and document the presence of microplastics, or tiny fragments of plastic, in aquatic ecosystems. The work stems from his Biofilms and Biodiversity curriculum, which engages students in project-based learning through the collection and observation of biofilm communities on discs submerged in the Baltimore Inner Harbor and in international waters including Sweden, Germany, and Spain.

These communities are now being examined for microplastics as well, and Frederick is testing new methods for classrooms to examine and catalog the particles. Our communications staff spent a day with him at IMET to test several different approaches for photographing samples.

A time-lapse sequence showing our communications team testing several different camera and lighting setups in the IMET laboratory. Credit: Lisa D. Tossey / MDSG

Our team tested several approaches, including extreme macro photography, using an objective lens mounted directly on a digital camera, as well as connecting the camera to two different types of microscopes. One was an inverted compound microscope with objectives mounted under the stage instead of above, which allows for a larger sample to be placed on the scope instead of just a slide. The other was a stereo, or dissecting, microscope, which allowed the capability of lighting with reflected lighting. That provided a more three-dimensional image that defines the material’s texture and color in a truer form.

Capturing a sample image using a 10x objective mounted directly on a mirrorless digital camera lens using an objective lens adapter. Photo credit: Lisa D. Tossey / MDSG

We placed samples in a glass Petri dish for viewing on the inverted compound microscope and then varied the objective from 4x to 20x magnification with transmitted light. It provided good depth of field and color reproduction, and overall is an excellent scope for the classroom to look at communities of biofilms, cells, protists, and hemocytes from oysters.

Nicole Lehming makes adjustments to the camera while photographing a biofilm sample in a Petri dish, at left. Photo credit: Lisa D. Tossey / MDSG

For the stereomicroscope, we used a 2x Barlow lens for macro work. Frederick feels that these scopes are the best choice to view other aspects of his biofilms and biodiversity work at IMET, as well as capturing images of the anatomy of organisms like oysters.

J. Adam Frederick examines the camera’s field of view using an external monitor. Photo credit: Lisa D. Tossey / MDSG

So what do microplastics look like under a scope? Here are a few of the resulting images captured by Frederick and Nicole Lehming, our graphic designer and producer. Overall, the team was happy with the results of their first round of testing, and Frederick will share details of the most successful techniques with the teachers who will be incorporating microplastics into their Biofilms and Biodiversity curriculum.

Microfiber on a filter paper sample, 4x magnification.
Microfiber and mussel byssal threads entangled, 10x magnification.
Fragment that may be from a plastic bag, 20x magnification.
Pink fragment that is possibly microplastic, intermixed with other cells and tube-like fragments that could be from a colonial bryozoan, 20x magnification.

Photo, top left: J. Adam Frederick reviews microscopic techniques with teachers at South Carroll High School. Credit: Wendy Mitman Clarke / MDSG

Macro photos taken by Nicole Lehming and J. Adam Frederick / MDSG.

 

About Maryland Sea Grant

Our communications staff works to inform the public about scientific discoveries and the researchers who make them, findings that can help to protect and restore the Bay and Maryland’s coastal waters. We strive to provide scientifically accurate information with context and depth that nonscientists can understand and enjoy.

Contact the author at communications@mdsg.umd.edu

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