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Stocked larvae of American shad contribute to adult stock in a restoration effort being conducted in Chesapeake Bay, but environmental conditions that optimize larval stocking procedures were unevaluated. For this research, larvae were stocked into the Patuxent River during 2000 and 2001 at prescribed locations and dates to determine preferred conditions that maximize growth, survival and interactions with other ichthyoplankton species. Recommended stocking strategies for resource management agencies will result from the research.