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This proposal continues and expands a project begun in 2000 involving the testing of a variety of technologies for ballast water treatment aboard a vessel in Baltimore Harbor owned by the U S. Maritime Administration. The focus of the current proposal is on combination technologies, specifically UV irradiation and biocide dosing (in series) of ballast water during a ballasting operation, primary filtration (using a depth filter & separator) used in tandem with UV irradiation and primary filtration, UV irradiation and biocide dosing used together.