Science Serving Maryland's Coasts

R/BT-08

Production of recombinant fish gonadotropins (GtHs) using eukaryotic expression systems and development of a striped bass gth immunoassay

Principal Investigator: 

Yonathan Zohar

Start/End Year: 

1998 to 2001

Institution: 

Center of Marine Biotechnology, University of Maryland Biotechnology Institute

Co-Principal investigator: 

B Weintraub, University of Maryland Biotechnology Institute, Medical Biotechology Center.; M Szkudlinski, University of Maryland Biotechnology Institute, Medical Biotechology Center

Topic(s): 

Description: 

To produce immunologically and biologically active recombinant striped bass GtH-I and GtH-II, and to develop an enzyme-linked immunosorbant assay (ELISA) for striped bass GtH-I. To use and optimize a novel and generic recombinant DNA approach for expressing biologically and immunologically active gonadotropins of fish, in view of enabling studies on their reproductive endocrinology.