Production of recombinant fish gonadotropins (GtHs) using eukaryotic expression systems and development of a striped bass gth immunoassay
Principal Investigator:Yonathan Zohar
Start/End Year:1998 to 2001
Institution:Center of Marine Biotechnology, University of Maryland Biotechnology Institute
Co-Principal investigator:B Weintraub, University of Maryland Biotechnology Institute, Medical Biotechology Center.; M Szkudlinski, University of Maryland Biotechnology Institute, Medical Biotechology Center
To produce immunologically and biologically active recombinant striped bass GtH-I and GtH-II, and to develop an enzyme-linked immunosorbant assay (ELISA) for striped bass GtH-I. To use and optimize a novel and generic recombinant DNA approach for expressing biologically and immunologically active gonadotropins of fish, in view of enabling studies on their reproductive endocrinology.