two oyster shells - top one showing inside and bottom showing outside of the shell

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Oysters & the
Chesapeake

 
Oyster Research and Restoration in U.S. Coastal Waters: Strategies for the Future
September 8-9, 2003 - Annapolis, Maryland

Abstracts
Workgroup: Frontiers in Disease Research

Development of Nuclear DNA Markers and Pedigreed Families for Disease Resistance and Genetic Mapping in the Eastern Oyster

Principal Investigator(s):
Patrick M. Gaffney, University of Delaware, pgaffney@udel.edu

Co-Investigator(s):
Standish K. Allen, Rutgers University
James C. Pierce, Philadelphia College of Pharmacy and Science

Funding Period: 1995

The bacteriophage P1 cloning system was used to construct a high-quality, high molecular weight genomic library. This is the first high molecular weight genomic library from a mollusk and should prove useful for a variety of purposes, particularly genetic mapping and isolation of genes or quantitative trait loci (QTL) affecting disease resistance in the eastern oyster.

Twenty-one single-pair matings of C. virginica were made. Tissues from all parents were archived. Juveniles from 14 families were raised at theRutgers Cape Shore Lab. These families have been used to construct second-generation crosses, to provide a two-generation pedigreed oyster archive for genetic mapping studies.

We developed additional nuclear DNA markers to supplement the currently available set of genetic markers.

IMPACTS and/or BENEFITS: Essential tools for genetic improvement of eastern oysters, i.e., DNA libraries, additional genetic markers, and pedigreed families were developed. These will contribute to the long-term goal of developing disease-resistant oysters for population restoration and aquaculture.

PROJECT PUBLICATIONS:

Gaffney, P. M., S. K. J. Allen, and J. Pierce. 1997. Development of nuclear DNA markers and pedigreed families for disease resistance and genetic mapping in the eastern oyster: Progress report. Journal of Shellfish Research 16:257.

Gaffney, P. M., E. A. Orbacz, and Z. Yu. 1998. Using the DCode system to identify DNA sequence variation for studies of population structure in marine organisms. Pp. 4. Bio-Rad.

Wakefield, J. R., and P. M. Gaffney. 1996. DGGE reveals additional population structure in American oyster (Crassostrea virginica) populations. Journal of Shellfish Research 15:513.

UM-SG-TS-2003-01 www.mdsg.umd.edu
   
This publication was supported by funds from
the NOAA National Sea Grant College Program and the
Maryland and Virginia Sea Grant College Programs
[Maryland Sea Grant]
 [NOAA]
 [Virginia Sea Grant]

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